<div dir="ltr"><div class="gmail_default" style="font-family:tahoma,sans-serif;color:rgb(102,102,102)">We have also recently found DWV in Bumble bees (in VA) but as John P comments, we cannot yet comment on pathogenicity. We are in the process of the quantification now.<br><br></div><div class="gmail_default" style="font-family:tahoma,sans-serif;color:rgb(102,102,102)">Lisa<br></div></div><div class="gmail_extra"><br><div class="gmail_quote">On Tue, Feb 9, 2016 at 10:29 AM, John Purdy <span dir="ltr"><<a href="mailto:johnrpurdy@gmail.com" target="_blank">johnrpurdy@gmail.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div dir="ltr">It would be good if those who announce the finding of viruses that are new or in new species could hold to a standard of science reporting that includes confirmation of both replication and pathogenicity. The first of these is easy once the RNA sequence is known but the second takes some effort. Viruses can be complicated. there is no merit in "crying wolf", but on the other hand we have experiences like Zika virus which was considered insignificant until the possibility of a major pathogenic outcome arose.<div><br></div><div>The other problems in working with viruses is the lack of diagnostic symptoms and the lack of quantitative thresholds for the results of molecular methods of detection. </div><div><br></div><div>For interest, I have attached a PDF of a USDA bulletin from 1914 that describes the symptoms of viruses. Viruses must have been a new phenomenon at the time,but the knowledge have been forgotten and rediscovered several times since then. Notice how similar they are to the symptoms described for pesticide poisoning. A troubling ambiguity.</div><div><br></div><div>John Purdy, PhD</div><div><br></div><div><br></div><div><br></div><div><br><div><br></div></div></div><div class="gmail_extra"><br><div class="gmail_quote"><div><div class="h5">On Mon, Feb 8, 2016 at 8:54 AM, Stoner, Kimberly <span dir="ltr"><<a href="mailto:Kimberly.Stoner@ct.gov" target="_blank">Kimberly.Stoner@ct.gov</a>></span> wrote:<br></div></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d">Hi Russel and others,<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d">Deformed wing virus and black queen cell virus have been found in some species of bumble bees here in the US. We collected Bombus impatiens and sent them off
to John Burand at the University of Massachusetts as part of a larger project and he found both of these viruses in our bumble bees from several different locations (pumpkin/squash fields) around Connecticut. Others have also found honey bee viruses in other
bees.<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d">Finding these viruses with PCR is not the same as showing that they replicate or are pathogenic in bumble bees. That has been shown with DWV and B. terrestris
in the UK, but not yet with US species, to my knowledge.<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1f497d">Kim<u></u><u></u></span></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <a href="mailto:beemonitoring@yahoogroups.com" target="_blank">beemonitoring@yahoogroups.com</a> [mailto:<a href="mailto:beemonitoring@yahoogroups.com" target="_blank">beemonitoring@yahoogroups.com</a>]
<b>On Behalf Of </b>Russel Barsh <a href="mailto:rlbarsh@gmail.com" target="_blank">rlbarsh@gmail.com</a> [beemonitoring]<br>
<b>Sent:</b> Saturday, February 06, 2016 11:40 PM<br>
<b>To:</b> Peter Bernhardt <<a href="mailto:bernhap2@slu.edu" target="_blank">bernhap2@slu.edu</a>><br>
<b>Cc:</b> Bee United <<a href="mailto:beemonitoring@yahoogroups.com" target="_blank">beemonitoring@yahoogroups.com</a>>; Pollinator List-serv <<a href="mailto:pollinator@lists.sonic.net" target="_blank">pollinator@lists.sonic.net</a>>; Graham Pyke <<a href="mailto:Graham.Pyke@uts.edu.au" target="_blank">Graham.Pyke@uts.edu.au</a>>; Gerardo Camilo <<a href="mailto:camilogr@slu.edu" target="_blank">camilogr@slu.edu</a>>; James Trager <<a href="mailto:James.Trager@mobot.org" target="_blank">James.Trager@mobot.org</a>><br>
<b>Subject:</b> Re: [beemonitoring] Scare Tactics?<u></u><u></u></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt">Peter --<u></u><u></u></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt">We read the underlying article in SCIENCE and yes, I think the press story you cited is implying that DWV is the sole or principal cause of colony collapse, and that its spread is a consequence of global bee-keeping.
The underlying article argues from phylogenetic data that DWV is an ancestral disease of honeybees, but that its transmission has been facilitated by Varroa mites as carriers. Living with a beekeeping and honey-making family (my in-laws on the islands here),
I hear stories all the time about sloppy inexperienced beekeepers that don't take proper measures to control mites. So the story may have been better titled "Sloppy beekeeping facilitates transmission of DWV worldwide".
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<p class="MsoNormal" style="margin-bottom:12.0pt">I'm much more disturbed by the evidence published in NATURE in 2014 for honeybee viruses jumping to bumblebees. We really need to do more collecting of wild bees and genomic screening for viruses such as DWV
that could be spread by domesticated and commercialized bees to wild species. <br>
<br>
I've been collecting wild herring (a whole different side of my science life) for colleagues at USGS here that are studying viral epidemics in fish that may be transmitted up the food chain i.e. from small fish to their predators. It's an angle I would like
to help launch in the study of pollinator networks.<u></u><u></u></p>
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<p class="MsoNormal">Thanks for throwing a spotlight on misleading reportage...<u></u><u></u></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt">Russel.<u></u><u></u></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt">Russel <u></u><u></u></p>
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<p class="MsoNormal">Russel Barsh<u></u><u></u></p>
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<p class="MsoNormal">Director, KWIAHT<u></u><u></u></p>
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<p class="MsoNormal">PO Box 415, Lopez Island WA 98261<u></u><u></u></p>
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<p class="MsoNormal">On Sat, Feb 6, 2016 at 3:18 AM, Peter Bernhardt <a href="mailto:bernhap2@slu.edu" target="_blank">
bernhap2@slu.edu</a> [beemonitoring] <<a href="mailto:beemonitoring-noreply@yahoogroups.com" target="_blank">beemonitoring-noreply@yahoogroups.com</a>> wrote:<u></u><u></u></p>
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<p class="MsoNormal" style="background:white">Please go to the following link. Do you think the media is overdoing it, just a bit, or is the situation as grim as they make it? Some sites are referring to the virus as a man-made (vs. man spread) disease.<u></u><u></u></p>
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<p class="MsoNormal" style="background:white"><a href="http://www.sciencedaily.com/releases/2016/02/160204150617.htm" target="_blank">http://www.sciencedaily.com/releases/2016/02/160204150617.htm</a><u></u><u></u></p>
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<p class="MsoNormal">Posted by: Russel Barsh <<a href="mailto:rlbarsh@gmail.com" target="_blank">rlbarsh@gmail.com</a>>
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<br></blockquote></div><span class="HOEnZb"><font color="#888888"><br><br clear="all"><div><br></div>-- <br><div><div dir="ltr">John Purdy PhD<div>Environmental Scientist</div><div>Abacus Consulting Services Ltd</div></div></div>
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<br></blockquote></div><br><br clear="all"><br>-- <br><div class="gmail_signature"><div dir="ltr"><div><div dir="ltr"><div><span style="font-family:tahoma,sans-serif"><font color="#999999"><br></font></span><div><span style="font-family:tahoma,sans-serif"><font color="#999999">Associate Professor<br>Dept of Biological Science<br>Old Dominion University<br>Norfolk, VA 23529</font></span></div></div><div><span style="font-family:tahoma,sans-serif"><font color="#999999"><a href="mailto:lhorth@odu.edu" target="_blank">lhorth@odu.edu</a></font></span></div></div></div></div></div>
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