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<span style="margin: 0px; font-weight: 400; font-size: 12pt; font-family: Calibri, Arial, Helvetica, sans-serif; color: black; background-color: rgb(255, 255, 255);">All: Am posting for Aaron Aunins, please reply directly to him.</span></div>
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<span style="margin: 0px; font-weight: 400; font-size: 12pt; font-family: Calibri, Arial, Helvetica, sans-serif; color: black; background-color: rgb(255, 255, 255);">"Aunins, Aaron W" <aaunins@usgs.gov><br>
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<span style="margin: 0px; font-weight: 400; font-size: 12pt; font-family: Calibri, Arial, Helvetica, sans-serif; color: black; background-color: rgb(255, 255, 255);">Feel free to repost, apologies for crosspostings </span></div>
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Greetings:</div>
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<span style="margin: 0px; font-weight: 400; font-size: 12pt; font-family: Calibri, Arial, Helvetica, sans-serif; color: black; background-color: rgb(255, 255, 255);">We are working with Nick Grishin's Lab at UT Southwestern for our eventual genomic analyses
of the Grizzled Skipper specimens we collect. Age of the specimens should not be of concern here, as his lab has worked successfully with 50+ year old specimens. We are requesting a whole leg removed with forceps and placed in an empty microcentrifuge tube
(no preservative like ethanol needed) for DNA extraction. The leg would be destructively sampled for DNA extraction and not returned to the entity that donated it. We can provide tubes and a Fedex account code for entities that wish to utilize that. Samples
would be returned to my mailing address in my signature. We are interested in the three subspecies:<span> </span><i>Pyrgus centaureae wyandot</i>,<span> </span><i>P. c. loki</i>, and<span> </span><i>P. c freija</i>. Our study is focusing on population structure
within the Appalachian Grizzled Skipper, but also the relationships among these subspecies. </span></div>
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Aaron</div>
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Aaron Aunins, Ph.D.</div>
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Research Fisheries Biologist</div>
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U.S. Geological Survey</div>
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Eastern Ecological Science Center at the Leetown Research Laboratory</div>
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<span style="margin: 0px; font-family: Calibri, Helvetica, sans-serif; background-color: white;">Species Population Dynamics & Surveillance Capability Team</span></div>
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11649 Leetown Road</div>
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Kearneysville, WV 25430</div>
<span style="margin: 0px; font-size: 12pt; font-family: Calibri, Arial, Helvetica, sans-serif; color: black;">Phone: (304)724-4480</span></div>
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<span style="color: rgb(15, 92, 26);">B</span><span style="color: rgb(15, 92, 26);"></span><span style="color: rgb(15, 92, 26);">e Kinder than Necessary</span></div>
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