[Pollinator] Wealth of orchid varieties is down to busy bees and helpful fungi

[Jennifer Tsang]

Thanks to Peter Loring Borst for forwarding the below:

http://www3.imperial.ac.uk/newsandeventspggrp/imperialcollege/newssummary/ne
ws_31-1-2011-17-29-17 
Monday 31 January 2011
For immediate release
Scientists have discovered why orchids are one of the most successful groups
of flowering plants - it is all down to their relationships with the bees
that pollinate them and the fungi that nourish them. The study, published
tomorrow in The American Naturalist <http://asnamnat.org/amnat> , is the
culmination of a ten-year research project in South Africa involving
researchers from Imperial College London, the Royal Botanic Gardens, Kew
<http://www.kew.org/> , and other international institutions.
The orchid family is one of the largest groups of flowering plants, with
over 22,000 species worldwide. Today's research suggests that there is such
a huge range of species because orchids are highly adaptable and individual
species can interact with  bees, and other pollinators, in different ways.
For example, when orchids Pterygodium pentherianum and Pterygodium schelpei
live side by side, Pterygodium pentherianum puts its pollen on the bee's
front legs, whereas Pterygodium schelpei puts it on the bee's abdomen, as in
the photo above. This means that one bee can carry pollen from two distinct
species without mixing it.
The study also shows how orchids are able to live harmoniously together,
with different species working in partnership with different microscopic
fungi in the soil, ensuring they do not compete with each other.
Prior to today's study, it was known that orchids have strong interactions
with bees, which pollinate the flowers in return for food such as nectar or
oils, and also with fungi, which supply minerals to the roots in return for
sugars. These relationships are amongst the best examples of nature's system
of 'mutual benefit' and are believed to have been important for enabling
orchids to evolve into so many different species. However, the mechanisms by
which these relationships affect the number of plant species, and these
species' ability to coexist, had remained obscure.
Professor Tim Barraclough <http://www3.imperial.ac.uk/people/t.barraclough>
, from the Division of Biology at Imperial College London, co-led an
international team of plant scientists to investigate how these interactions
affect orchid diversity.  "Orchids are hyper-diverse globally, particularly
in South Africa, where they have diversified to a large extent, so we wanted
to find out how lots of species are able to exist without competition," he
said.

The oil-secreting orchid Pterygodium halii, uses unique scent to attract
female oil-collecting bees of the species Rediviva macgregori (Copyright A
Pauw)
The group studied 52 orchid species in a small region of South Africa, which
all secrete oil inside their flowers that female bees collect to feed to
their larvae. In order to investigate which pollinating bees were visiting
the different species, they collected orchid pollen from the bees for DNA
sequencing and analysis. They found strong evidence that when an orchid
moved to a new geographical area it adapted to a different pollinating bee
species, and interestingly, some competing orchid species were able to adapt
by placing pollen on different body parts of the same bee.
"What is remarkable in these orchids is that diversity is generated not only
through switches between bees, but also by switches between different body
parts of the same bee, so two closely related orchids might place pollen on
different segments of one bee's front leg," added Professor Barraclough.
"It's given us a fundamental insight into how so many new species can
originate, and once they originate how they are able to coexist without
exchanging genes."
The researchers also studied the microscopic fungi living on the roots of
the orchid, to see how this relationship was affecting plant diversity. Most
flowering plants host microscopic fungi in their roots that help the plant
take up nutrients from the soil. Until now it has been difficult to
investigate this interaction, as most of the fungi belong to species that
are difficult to culture. The researchers overcame this challenge by
combining a molecular technique known as DNA barcoding with field
experiments. In contrast to the bees, where co-occurring orchid species
normally share the same insect pollinator, the plants needed to use
different fungal partners in order to coexist in the same region.
"By tapping into different kinds of fungi, different plant species access
different pools of nutrients and so the problem of living together without
competing for the same resources is solved," said Professor Barraclough.
However, the same fungal partners are found in different geographical areas
and so orchid species that originate in different areas, by adapting to
different pollinators, tend still to use the same fungi.
The team's fieldwork shows that shifts in pollination traits were important
for bringing about new species and allowing coexistence in a diverse group
of orchids, whereas shifts in fungal partner were important for coexistence
but not for speciation. Many other groups of flowering plants enter into
similar relationships with pollinators and fungi, and both the origins and
the future survival of that diversity could depend critically on
understanding these relationships.
Dr Richard Waterman, now at th e University of Sheffield, who conducted the
research as part of his PhD at Imperial College London and the Royal Botanic
Gardens, Kew, commented on the next steps for the scientists: "We need a
better understanding of these relationships if we are to predict and counter
the effects of the worldwide decline in pollinators and soi l quality."
The r esearch was funded by the Natural Environment Research Council (NERC)
<http://www.nerc.ac.uk/> , NRF South Africa <http://www.nrf.ac.za/> ,
Stellenbosch University <http://www.sun.ac.za/> , NSF IGERT
<http://www.nsf.gov/crssprgm/igert/intro.jsp>  and the Royal Botanic
Gardens, Kew <http://www.kew.org/> . 
For further information please contact:
Simon Levey 
Research Media Officer 
Imperial College London 
email: s.levey at imperial.ac.uk  <mailto:s.levey at imperial.ac.uk> 
Tel: +44(0)20 7594 6702 
Out of hours duty press officer: +44(0)7803 886 248
Notes to editors:
1. Waterman RJ et al. "The effects of above and below ground mutualisms on
orchid speciation and coexistence" as published in The American Naturalist,
1 February 2011. [ref/DOI]. For a full list of authors please refer to the
paper. DOI: 10.1086/657955 http://www.jstor.org/stable/10.1086/657955
2. Images:
(1) Several kinds of oil-secreting orchids share the same pollinator by
placing pollen on different parts of the bee's body. Here the long-legged
oil-collecting bee Rediviva longimanus inspects the oil-secreting orchid
Pterygodium pentherianum. (Copyright A Pauw)
https://fileexchange.imperial.ac.uk/files/867594b0467/Fig%201b2%20(copyright
%20A%20Pauw).jpg
<https://fileexchange.imperial.ac.uk/files/867594b0467/Fig%201b2%20%28copyri
ght%20A%20Pauw%29.jpg> 
(2) Rediviva macgregori & Pterygodium halii. The oil-secreting orchid
Pterygodium halii, uses unique scent to attract female oil-collecting bees
of the species Rediviva macgregori. (Copyright A Pauw)
https://fileexchange.imperial.ac.uk/files/c73ba586d10/Fig%202%20(Copyright%2
0A%20Pauw).jpg
<https://fileexchange.imperial.ac.uk/files/c73ba586d10/Fig%202%20%28Copyrigh
t%20A%20Pauw%29.jpg> 
(3) The oil-collecting bee Rediviva longimanus delves deep into the orchid
Pterygodium schelpei to extract oil with absorbent hairs on its elongated
front feet.(Copyright A Pauw)
https://fileexchange.imperial.ac.uk/files/8347b80778/Fig%203%20(Copyright%20
A%20Pauw).jpg
<https://fileexchange.imperial.ac.uk/files/8347b80778/Fig%203%20%28Copyright
%20A%20Pauw%29.jpg> 
3. The Royal Botanic Gardens, Kew is a world famous scientific organisation,
internationally respected for its outstanding living collection of plants
and world-class Herbarium and Laboratory as well as its scientific expertise
in plant diversity, conservation and sustainable development in the UK and
around the world. Kew Gardens is a major international visitor attraction.
Its landscaped 132 hectares and RBG Kew's country estate, Wakehurst Place,
attract nearly 2 million visitors every year. Kew was made a UNESCO World
Heritage Site in July 2003 and celebrated its 250th anniversary in 2009.
Wakehurst Place is home to Kew's Millennium Seed Bank, the largest wild
plant seed bank in the world. RBG Kew and its partners have collected and
conserved seed from 10% of the world's wild flowering plant species (c.30,
000 species) and aim to conserve 25% by 2020. http://www.kew.org
4. About Imperial College London
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